Paramax triglycerides reagent: interference from high L-lactate and lactate dehydrogenase

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The mitochondrial L-lactate dehydrogenase affair

The existence of a mitochondrial L-lactate dehydrogenase (m-L-LDH) suggested by Dianzani (1951), was shown by Baba and Sharma (1971) with the enzyme located in the mitochondrial matrix; later Brooks et al. (1999) proposed the intracellular lactate shuttle and in the third millennium the existence of m-L-LDH was definitively been confirmed in mammalian, plant and yeast mitochondria as reviewed b...

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Inducible Membrane-bound L-Lactate Dehydrogenase from Escherichia coZi

Membrane-bound L-lactate dehydrogenase was solubilized from the membranes ofEscherichia coli and purified to homogeneity using conventional procedures. The enzyme had a pH optimum of 8 to 9 and was specific for L-lactate. Its apparent K, for L-lactate and maximal velocity were 1.2 x lo-’ M and 31 pmol of tetrazolium dye reducedlminlmg of protein, respectively. It had a polypeptide molecular wei...

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Characterization of the L-Lactate Dehydrogenase from Aggregatibacter actinomycetemcomitans

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LACTATE DEHYDROGENASE Elevation ofserum lactate dehydrogenasecommences

Heart tissue injury may release cardiac enzymes into the circulation and elevate serum enzyme levels (LaDue, Wroblewski, and Karmen, 1954). Many enzymes become raised, but three enzymesaspartate aminotransferase (EC 2.6.1.1), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27)-have proved of particular diagnostic value. In addition, determination of lactate dehydrogenase isoen...

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NAD-Independent L-Lactate Dehydrogenase Is Required for L-Lactate Utilization in Pseudomonas stutzeri SDM

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1996

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/42.5.778